Genetic variability and forensic efficiency of 39 microsatellite loci in the Li ethnic group from Hainan Island in the South China Sea

Abstract

Background: Investigation of allele and genotype frequencies of microsatellite loci in various populations is an essential pre-requisite in forensic application.

Aim: The present study obtained population genetic data and forensic parameters of 39 autosomal Short Tandem Repeats (STRs) loci from a Chinese Li ethnic group and estimated the genetic relationships between Li and other reference populations.

Subjects and methods: Thirty-nine STR loci, which include D19S433, D5S818, D21S11, D18S51, D6S1043, D3S1358, D13S317, D7S820, D16S539, CSF1PO, Penta D, D2S441, vWA, D8S1179, TPOX, Penta E, TH01, D12S391, D2S1338, FGA, D6S477, D18S535, D19S253, D15S659, D11S2368, D20S470, D1S1656, D22-GATA198B05, D8S1132, D4S2366, D21S1270, D13S325, D9S925, D3S3045, D14S608, D10S1435, D7S3048, D17S1290 and D5S2500, were amplified in two multiplex DNA-STR fluorescence detection systems for 189 unrelated healthy individuals of the Chinese Li ethnic group. The allele frequency distribution and several parameters commonly used in forensic science were statistically analysed.

Results: A total of 378 alleles were observed with corresponding allelic frequencies ranging from 0.0026–0.5899. The power of discrimination and power of exclusion ranged from 0.7569–0.9672 and 0.2513–0.7355, respectively. The power of exclusion (PE) ranged from 0.2580–0.7943 for trio paternity cases and 0.1693–0.5940 for duo paternity cases. The polymorphism information content (PIC) ranged from 0.5001–0.8611. The cumulative match probability across these 39 loci was 2.4242?×?10^?38.

Conclusion: The results indicate that 39 STR loci are polymorphic among the Li ethnic group in Hainan Island in the South China Sea. This set of polymorphic STR loci provide highly polymorphic information and forensic efficiency for forensic individual identification and paternity testing, as well as basic population data for population genetics and anthropological research.     

基于网络药理学的大果木姜子油治疗心力衰竭作用机制研究

目的:揭示大果木姜子油治疗心力衰竭的作用机制。方法:首先从动物药理学实验验证疗效,采用异丙肾上腺素(ISO)诱导复制心力衰竭大鼠模型,造模成功后应用大果木姜子油治疗4周,监测血流动力学指标,分析血清BNP水平和心肌组织形态学变化;然后基于文献检索和TCMSP数据库获取大果木姜子油活性成分及作用靶点,从CTD和Drugbank数据库筛选心力衰竭的已知治疗靶点,将疾病治疗靶点与大果木姜子油活性成分作用靶点进行Venn分析,运用SYBYL软件进行分子对接验证,运用STRING数据库分析蛋白互作关系,再利用DAVID数据库进行GO分子功能和KEGG通路富集分析。结果:大果木姜子油可显著升高ISO诱导的心衰大鼠LVSP、±dp/dtmax,降低LVEDP和BNP水平,改善心肌组织形态学变化;获得大果木姜子油20个活性成分,其中7个活性成分(沉香螺旋醇、芳樟醇、萜品烯-4-醇等)可能通过调控一个蛋白互作网络治疗心力衰竭,该网络中的靶点有PTGS1、PTGS2、NOS2、NOS3、GSK3B;由蛋白富集分析发现,这些靶点与前列腺素内过氧化物合酶活性和一氧化氮合酶活性有关。结论:大果木姜子油可改善心衰大鼠的血流动力学紊乱及心肌组织形态学变化;其治疗心力衰竭的机制可能与调控前列腺素内过氧化物合酶活性和一氧化氮合酶活性有关。     

李庆海从络、脾、肾治疗心悸

李庆海教授在临床中主张心悸治络、心悸醒脾、心悸治肾。心悸治络:对因虚致心悸者,用益气养血充络之法,气足则血旺而运行有力,血足则络得充养而心神得藏。常用黄芪、人参、阿胶。大剂量黄芪益气养血,补扶虚损。心悸醒脾:脾喜燥而恶湿,土爱暖而喜芳香,通过温里、补益、芳香等药物醒脾、健脾、运脾,醒复脾之神机。常用脾脏第一要药白术,配合人参、炙甘草、茯苓益气健脾。心悸治肾:治疗反复发作久治不愈的心悸时,从肾着手,予滋肾填精、温肾助阳药治之,常用熟地黄,山萸肉、巴戟天。     

电感耦合等离子体发射光谱（ICP）检测电生理导管可沥滤物的试验方法研究

本文采用电感耦合等离子体发射光谱法检测电生理导管中四种可沥滤物：硫酸钡,颜料黄53,颜料棕24和二氧化钛的含量。经方法验证,该方法校准曲线线性良好,线性范围达4~6个数量级;检出限极低,小于0.1μg/mL;回收率达到95%~103%;实验室内检测变异系数小于3%。该方法可同步测量多种超低痕量金属元素,大大减少了样品预处理和样品使用量,具有实用性和经济性,值得推广使用。     

黎氏哮喘I号方治疗小儿外寒肺热型哮喘50例疗效观察

目的观察黎氏哮喘I号方治疗小儿外寒肺热型哮喘的临床疗效。方法将80例哮喘患儿随机分为2组。治疗组50例口服黎氏哮喘I号方加减治疗;对照组30例予以西医抗炎、平喘药物治疗。2组疗程均为7d。结果治疗组临床治愈38例,好转10例,未愈2例,总有效率为96．0％;对照组临床治愈9例,好转16例,未愈5例,总有效率为83．3％。2组总有效率比较,差异有统计意义（P〈0．05）。2组治疗前后同组症状积分值比较,差异有统计意义（P〈0．05或P〈0．01）;治疗后与对照组比较,差异有统计意义（P〈0．05）,治疗组优于对照组。结论黎氏哮喘I号方治疗小儿外寒肺热型哮喘可明显改善患儿临床症状,疗效显著。     

长药隔重楼化学成分研究

目的 研究长药隔重楼Paris polyphylla var. pseudothibetica根茎中的化学成分,为阐明其有效成分及扩大重楼属植物的药用资源提供科学依据。方法 利用正相硅胶柱色谱、葡聚糖凝胶Sephadex LH-20、正反相制备色谱等手段进行分离纯化,并通过¹H-NMR、¹³C-NMR、ESI-MS等波谱技术进行结构鉴定。结果 从长药隔重楼中分离得到了12个化合物,分别鉴定为：薯蓣皂苷元（1）、薯蓣皂苷元-3-O-α-L-呋喃阿拉伯糖基-(1→4)-[α-L-吡喃鼠李糖基-(1→2)]-β-D-吡喃葡萄糖苷（2）、偏诺皂苷元-3-O-α-L-呋喃阿拉伯糖基-(1→4)-β-D-吡喃葡萄糖苷（3）、偏诺皂苷元-3-O-α-L-呋喃阿拉伯糖基-(1→4)-[α-L-吡喃鼠李糖基-(1→2)]-β-D-吡喃葡萄糖苷（4）、偏诺皂苷元-3-O-α-L-吡喃鼠李糖基-(1→4)-α-L-吡喃鼠李糖基-(1→4)-[α-L-吡喃鼠李糖基-(1→2)]-β-D-吡喃葡萄糖苷（5）、β-谷甾醇（6）、豆甾醇（7）、胡萝卜苷（8）、豆甾醇-3-O-β-D-吡喃葡萄糖苷（9）、山柰酚（10）、β-蜕皮激素（11）、蔗糖（12）。结论 化合物1～9为首次从该植物中分离得到。     

Sensitivity to H, Li and Mg ions of the slow inward sodium current in frog atrial fibres.

Membrane currents have beenmeasured in frog atrial fibres under voltage clamp conditions (double sucrose gap method); the effects on these currents of (i) an increase in [H]_o, (ii) the complete substitution of external Na by Li, (iii) the addition of Mg have been studied.The TTX-sensitive rapid inward current is decreased in acid Ringer (pH 5.6) as compared to values in normal Ringer (pH 7.8); it is unchanged in Li-Ringer and increased in Mg-Ringer. The slow inward calcium current (measured in TTX, Na-free solution) is decreased in acid Ringer and in Mg-Ringer and is unchanged in Li-Ringer. Increases or decreases affecting the rapid sodium current and the calcium current never exceed 40% and are generally smaller. On the other hand the slow inward sodium current (measured in TTX, Ca-free solution) is always suppressed in the three conditions studied.The participation of Na ions to the slow inward current in so called normal Ringer, that is in Mg-free Ringer is discussed. Indirect evidences are presented in favour of such a participation. After addition of Mg 1.8 mm to Ringer the slow inward current is carried only by Ca ions. Since the slow inward sodium current increases markedly in Mg-free Ca-free Ringer, it is concluded that the slow Na conductance is controlled by both Ca and Mg ions in addition to being strongly pH-dependent. It is also suggested that Na and Ca ions participating to the slow inward current do not penetrate necessarily through the same channel.     

“芎䓖”“蘪芜”“江蓠”“蓠”名实考证

古代辞书中保存了大量药名,后世学者在旧注中对药物的名实关系进行了细致考证。“芎䓖”“蘪芜”“江蓠”“蓠”形态特征、气味相似,难以分清,且文献中的记载较为混乱。现对“芎䓖”“蘪芜”“江蓠”“蓠”进行考证,利用《说文解字》《尔雅》等古代辞书与注家旧注,结合相关文献,进行整理分析,并以此补正本草文献内容。